Abstract: On March 28, 2025, a M_S7.9 earthquake occurred in Myanmar. Most of the fluid observation wells in the jurisdiction of Tengchong Central Station recorded significant co-seismic response characteristics. By analyzing the observation data of water temperature and water level at Tengchong Central Station, the dynamic response law of underground fluid under earthquake triggering is revealed. The results show that the co-seismic response of water level presents multiple forms, among which three wells show step-up change, two wells show oscillating response, and one well shows oscillating characteristics after step-down, reflecting the pore pressure fluctuation and hydrodynamic exchange process caused by seismic waves. The water temperature response is mainly step-up and oscillating, and the response signals of two other wells are weak, indicating that the thermal signal is less sensitive to ground motion than the pressure signal. Further research shows that the co-seismic response ability of water level and water temperature observation at Tengchong Central Station is significantly different. The water temperature response is controlled by the heat exchange process of the water body, showing a lag of 1-2 minutes and complex recovery characteristics ; the co-seismic response of well water level is mainly restricted by geological tectonic environment and hydrogeological conditions, and the water temperature response mechanism involves the coupling of multiple factors such as seismic wave-induced hydrodynamic model, thermal convection effect and sensor layout parameters, and its mechanism is more complex. Through the analysis of the co-seismic response characteristics of water temperature and water level in the area, it can provide a theoretical basis for the optimal layout of fluid wells and the identification of abnormal signals of underground fluid.